|
KMID : 1145120160140020127
|
|
Asian Journal of Beauty and Cosmetology
2016 Volume.14 No. 2 p.127 ~ p.137
|
|
|
β-Glucan Contents and Anti-wrinkling Effects of Brown Rice Phellinus linteus Mycelium Extracts Fermented with Lactobacillus plantarum
|
|
Kim Jae-Won
Ahn Jung-Mo
Kwon O-Jun
|
|
|
Abstract
|
|
|
Purpose: This study aimed to investigate the potential of brown rice Phellinus linteus as an anti-wrinkle material for cosmetics through examining anti-wrinkle effects.
Methods: To check the anti-wrinkle effects of brown rice Phellinus linteus mycelium, we performed various experiments such as β-glucan content, β-glucan molecular weight, cell viability, matrix metalloproteinase-1 (MMP-1) inhibitory activity, and pro-collagen synthesis assay.
Results: The β-glucan contents from brown rice Phellinus linteus mycelium extracts unfermented and fermented were 8.1, 12.8 g/100 g, rose up respectively and the fermented increased 58% compared with unfermented extract. The low molecular weights of the β-glucan such as less than 2500 m/z from the fermented was measured by MALDI-TOF mass spectrometry. We found that high molecular weight of β-glucan decomposed low molecular weight through fermentation. Measuring cytotoxicity of brown rice Phellinus linteus extracts in HS27 cells, cytotoxicity of both unfermented and fermented was not observed under 1,500 μg/mL concentration. After irradiating UVB treatment, HS27 cell viability of unfermented and fermented both showed the highest effect at 800 μg/mL concentration. The inhibitory activity of collagenase (MMP-1) of unfermented and fermented both showed the highest effect at a concentration of 400 μg/mL and the inhibitory activity of collagenase (MMP-1) of the fermented at a concentration of 1000 μg/mL had similar effects with adenosine at a concentration of 400 μg/mL. The amounts of biosynthesis of pro-collagen type I of the unfermented and fermented both increased more than three times compared with those of control.
Conclusion: Through results, we identified anti-wrinkle effects of the brown rice Phellinus linteus and we anticipated the brown rice Phellinus linteus develop the functional cosmetic ingredient as anti-wrinkle effects.
|
|
|
KEYWORD
|
|
|
Anti-wrinkle, Brown rice, Phellinus linteus, Lactobacillus plantarum, Β-glucan
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|